Human oral keratinocytes
Web25 Sep 2024 · A widely used immortalized human oral keratinocyte cell line is OKF6/TERT2, which originates from the floor of the mouth. A standardized protocol of OKF6/TERT2 cells cultured on fibroblasts-populated collagen gels, grown at the air-liquid interface, has been published previously. Citation 281 WebThe melanins are then transferred from melanocytes to keratinocytes, where they accumulate perinuclearly in membrane-bound organelles as a… Mostrar más Abstract Human skin and hair pigmentation play important roles in social behavior but also in photoprotection from the harmful effects of ultraviolet light.
Human oral keratinocytes
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WebExtracellular Vesicles and Their Zeta Potential as Future Markers Associated with Nutrition and Molecular Biomarkers in Breast Cancer WebKeratinocytes cover both the skin and some oral mucosa, but the morphology of each tissue and the behavior of the keratinocytes from these two sites are different. One …
WebIn this chapter, we describe an in vitro assay to characterize the response of human oral keratinocytes (OKF6/TERT-2) to the presence of the human pathogenic fungus, Candida albicans. The dynamic cellular changes such as expression of differentiation markers can be monitored by epifluorescence deconvolution microscopy. Analyses of ... Web22 May 2024 · The effect of Elental® on the growth and migration ability of the human oral keratinocyte cell line, HOK, was also examined using MTT and migration assays, respectively. In the mouse model, both oral administration and topical application of Elental® reduced 5‑FU‑induced dermatitis and healed raw wound areas more effectively …
WebHuman oral keratinocyte (HOK) cells were acquired by Cell Bank of the Chinese Academy of Sciences (Shanghai, China), and maintained in Keratinocyte-SFM medium (Invitrogen, Carlsbad, CA, USA). OSCC cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Carlsbad, CA, USA) with 10% fetal bovine serum (FBS; Invitrogen) and … Web25 Jul 2024 · Among the up-regulated transcripts in the oral samples, we found genes described in wound-activated keratinocytes ( 6 ), including keratins 6 ( KRT6) and 16 ( KRT16 ), small proline-rich ( SPRR) and S100 proteins, defensins, serpins, and annexins among others ( Fig. 2C ).
WebThe present study showed that guava mouth rinse fulfilled the requirement for an effective and useful oral care product with desirable substantivity and anti-biofilm action. ... (0.15%) and CHX in human gingival epithelial keratinocytes (HGEK-16). Cells were treated with lipopolysaccharides (LPS, 1μg/mL) and guava to assess inflammatory gene ...
WebPsoriasis is a multifactorial, chronic inflammatory skin disease, the development of which is affected by both genetic and environmental factors. Cytosolic nucleic acid fragments, recognized as pathogen- and danger-associated molecular patterns, are highly abundant in psoriatic skin. It is known that psoriatic skin exhibits increased levels of IL-23 compared … banca di bari loginWebTelomerase activity was analyzed in 7 different cultures of secondary normal human oral keratinocytes (NHOKs), 1 normal human oral epithelial tissue specimen, 1 … banca di asti bergamoWeb4 Feb 2014 · Human oral keratinocyte (OK) cells (5000 cells/well) seeded in 96-well plates were incubated in medium containing 2% FBS overnight. Then, the cells were further cultured for 6, 12, or 24 hours after replacement of culture fluids with fresh medium containing 10% FBS and the test sample. Cell metabolic activities were measured using … banca di asti remote bankingWeb10 Mar 2024 · The oxygen concentration in normal human tissue under physiologic conditions is lower than the atmospheric oxygen concentration. The more hypoxic condition has been observed in the cells with wound healing and cancer. Somatic stem cells reside in a hypoxic microenvironment in vivo and prefer hypoxic culture conditions in vitro. Oral … arti aos dalam bahasa jawaWebDescription. Human Oral Keratinocytes (HOK) from Creative Bioarray are isolated from human oral mucosa. HOK are cryopreserved on passage one culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HOK are characterized by immunofluorescent method with antibodies to cytokeratine-8, -18 and -19. banca di asti wikipediaWeb21 Jun 2014 · Immortalized normal human oral keratinocytes (OKF6/TERT-2) [ 22] obtained from Dr James Rheinwald (Brigham and Women’s Hospital, Boston, USA), were seeded into culture dishes in serum-free keratinocyte medium (Gibco) supplemented with 0.2 ng ml -1 human recombinant epidermal growth factor, 25 μg bovine pituitary extract … banca di asti mailWebHuman Caucasian dysplastic oral keratinocyte The dysplastic oral keratinocyte cell line DOK was isolated from a piece of dorsal tongue from a 57 year old man. After a squamous-cell carcinoma was removed from the patient, who had been a heavy smoker, the remaining dysplasia was removed subsequently and used to initiate primary cultures leading to the … arti aos dalam judi bola