WebTHP-1 is a human monocytic cell line derived from an acute monocytic leukemia patient. It is used to test leukemia cell lines in immunocytochemical analysis of protein-protein … WebTHP-1 and U937 monocytic cells were cultured in medium supplemented ... Development of in vitro assays using macrophage-like cell lines for the screening of novel anti-cancer …
Antibody-Dependent THP-1 Cell-Mediated Phagocytosis of
WebApr 18, 2024 · Some might argue that the clearance cue is the senescence signal that leads to RBC phagocytosis after circulating weeks (in mouse) or months (in human), but CD47 … WebRoswell Park Memorial Institute (RPMI) 1640 medium is a commonly used medium for THP-1 as well as for U937 cells. Alternatively, DMEM (Dulbecco’s Modified Eagle’s Medium; Morton 1970), also supplemented with 10 % FBS, is used to grow U937 cells.RPMI 1640 medium was originally developed by Moore et al. (), at Roswell Park Memorial Institute to … rockwall hud
Plasticity of Human THP-1 Cell Phagocytic Activity during
WebMay 30, 2024 · Phagocytosis Assays. THP-1 and WBC264-9C cell lines were from ATCC (Manassas, VA, USA). Human umbilical vein endothelial cells (HUVECs) were from … WebVisualize morphology and cell surface markers, to study differentiation. THP-1 monocytes were exposed to media (undifferentiated), vitamin D3 or PMA in the presence of Incucyte … WebImportantly, M2 surface markers of THP-1-derived macrophages were induced by recombinant MIF1 and reduced by using MIF1 inhibitor (S,R)-3-(4-hHydroxyphenyl)-4,5-dihydro-5-isoxazole acetic acid (ISO-1). Furthermore, GBM tumor model data suggested that the tumor growth, ... 4.11. Phagocytosis Assay. ottawa university law school admissions